Development of an Effective DNA Vaccine Against Viral Edema of Carp/Koi Sleepy Disease Caused by Carp Edema Virus

The authors declare no conflicts of interest. The data that support the findings of this study are available from the corresponding author upon reasonable request. Figure S1: Phylogenetic tree analysis of the P4a gene nucleotide sequence among carp edema virus isolates. This phylogenetic tree was constructed using the maximum likelihood (ML) method in MEGA12. Each isolate deposited in GenBank is shown with its accession number and country of origin in parentheses. The isolate NG202001 (Acc. No. LC896241) used in this study is enclosed in a red box. Bootstrap values (%) from 1000 trials are shown within each node (values below 60% are not shown). Figure S2: Construct of the DNA vaccine pcDNA3.1_ORF173 and pcDNA3.1_ORF174. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.