A surrogate‐selection‐enhanced TnpBmax platform enables efficient genome editing in rice and tomato

Journal of Integrative Plant Biology 2026 Open access

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Authors: E. F. Li, Jiyong Xie, Xinbo Li, Zai Zheng, Mugui Wang, Jian‐Kang Zhu, Yifu Tian
DOI: 10.1111/jipb.70159
Cited by: 2
References: 10

Abstract

TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.

Fields of Study & Subjects

CRISPR and Genetic Engineering RNA regulation and disease RNA and protein synthesis mechanisms

Publication details

Journal / source
Journal of Integrative Plant Biology
Publisher
Wiley
Volume
68
Issue
9
Pages
3166–3168
Publication date
28 January 2026
Language
EN