Evaluation of a novel anti‐mycotoxin agent specific for aquatic species in mitigating aflatoxin B1 ‐induced changes in Nile tilapia: A comprehensive study

Abstract Aflatoxin B1 (AFB1) contamination of aquaculture feed poses serious health risks to fish and consumers. In this study, the efficacy of a novel anti‐mycotoxin agent specific for aquatic species (AM‐AQ) containing bentonite, sepiolite, and orange peel meal ( Citrus Sinensis ) in alleviating AFB1 toxicity in Nile tilapia ( Oreochromis niloticus ) was evaluated. Fish were fed a basal diet as a control group and diets containing AFB1 (50 μg/kg), AM‐AQ (3 g/kg), or a combination of both for 6 weeks. AFB1 exposure significantly reduced growth, altered hematological parameters, protein profile, and digestive enzyme activities ( p < 0.05), and suppressed respiratory burst activity ( p < 0.001). It also altered the expression of liver and kidney function enzymes, lysozyme, and pro‐inflammatory cytokines and generated oxidative stress ( p < 0.05, p < 0.01). AM‐AQ supplementation effectively counteracted these adverse effects by restoring these parameters to control levels. Notably, AM‐AQ reduced AFB1 residues in the musculature by 99.6% after 6 weeks ( p < 0.001). Histopathological analysis revealed severe liver, spleen, and intestinal damage in the group exposed to AFB1, which was markedly ameliorated by AM‐AQ treatment ( p < 0.05). These results demonstrate that the AM‐AQ is a promising feed additive for protecting Nile tilapia against AFB1 toxicity and for reducing AFB1 transfer to edible tissues. Further research is required to optimize its application in aquaculture.