Stomata as a Defence Nexus: Integrating Drought and Pathogen Stress Signalling

ABSTRACT Plant stomata are microscopic leaf pores that regulate gas exchange but also serve as pathogen entry points and frontline sensors for stresses like drought. This review synthesises current understanding of stomata's dual defensive roles in coordinating responses to pathogen invasion and drought stress, highlighting sophisticated synergistic and antagonistic mechanisms governing stomatal dynamics under combined pressures. Guard cells integrate diverse stress signals through complex, overlapping signalling networks involving mitogen‐activated protein kinase cascades, reactive oxygen species bursts, Ca 2+ oscillations, and small signalling peptides. Functioning as pivotal nodes in the evolutionary 'arms race' between plants and pathogens, stomata exhibit significant crosstalk between abscisic acid‐mediated drought responses and pathogen‐associated molecular patterns‐triggered immunity. Drought‐induced closure can paradoxically create pathogen‐favourable microenvironments, while pathogen signals modulate long‐term stomatal development. Critical unresolved questions concern defence‐growth trade‐offs, signalling pathway interference, and tissue‐specific functional redundancy. Cutting‐edge techniques like single‐cell sequencing and synthetic biology are revolutionising understanding and enabling engineering approaches. Recognising stomata as central hubs for hierarchical environmental adaptation is crucial for developing strategies to enhance crop resilience against increasing compound stresses under climate change, particularly through engineering optimised stomatal responses for concurrent drought tolerance and disease resistance.