A Multithermic Machine Perfusion System Capable of Precise Control of Perfusion Parameters and Stepwise Loading of Cryoprotectants for Mutiscale Organ Preservation

Abstract Organ transplantation is the only effective therapeutic modality for end-stage organ diseases. However, short preservation windows of traditional static cold storage (several hours) and donor scarcity limit the development of transplantation. Machine perfusion (MP) has emerged as a superior preservation and repair strategy. It has been demonstrated that a reduction in temperature can further diminish organ metabolic rates, thus minimizing consumptive injury. Currently, machine perfusion devices for organs above 0 °C are available, but those for subzero temperatures and suitable for multiple organs remain underdeveloped. Here, we develop a multithermic machine perfusion (MTMP) system, which enables programmable and precise regulation over a wide temperature range from normothermia (37 °C), hypothermia (4 °C) to supercooling (