Superresolution imaging of live samples by centroid reassignment microscopy
Abstract
We describe a technique of superresolution microscopy using a variation on confocal microscopy (CRM) that increases spatial resolution, light collection efficiency and robustness to aberrations. Our technique, called centroid reassignment microscopy, is a simplified version of image scanning microscopy (ISM) with improved performance. Because CRM operates with conventional fluorophores and labeling protocols, it is well suited for dynamic, live-cell imaging.
Fields of Study & Subjects
Advanced Fluorescence Microscopy Techniques
Cell Image Analysis Techniques
Advanced Image Processing Techniques
Publication details
- Journal / source
- Single Molecule Spectroscopy and Superresolution Imaging
- Pages
- 15–15
- Publication date
- 4 March 2026
- Language
- EN