Calibration methods for single-molecule microscopy

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing 2026

View at publisher
Authors: Yi-Ting Sherry Feng, Luis A. Alemán-Castañeda, Thomas G. Brown, Miguel Angel Alonso
DOI: 10.1117/12.3079398
Cited by: 0

Abstract

Precise calibration is critical for reliable determination of fluorophore orientation and localization in single-molecule microscopy. Random nanoparticles on interfaces can be excited as dipoles, mimicking single molecules, but careful arrangement is still required. Here, we demonstrate a proof of concept for generating out-of-plane dipoles under a commercial microscope for reliable microscope calibration.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Force Microscopy Techniques and Applications Orbital Angular Momentum in Optics

Publication details

Journal / source
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing
Pages
19–19
Publication date
4 March 2026
Language
EN