FLIM playground: an interactive, end-to-end graphical user interface for analyzing single cell fluorescence lifetime imaging microscopy

FLIM Playground is an open source, cross platform, Python based interactive graphical platform that unifies all stages of fluorescence lifetime imaging microscopy (FLIM) workflows. The Data Extraction module organizes image metadata, calibrates instrument response function (IRF) shift, extracts single-cell features including fitted, fit-free and others, merges datasets, and assigns categorical labels to each cell. Next, the Visualization and Classification modules offer diverse analysis methods, including dimension reduction, heterogeneity analysis, and machine learning classifiers. The platform offers interactive widgets to enable code free, real time navigation and exploration of large datasets. By adopting best practices and offering flexibilities through interactivity, FLIM Playground accelerates hypothesis driven discovery and promotes reproducibility.