Two-photon diagnosis of dermatological biopsies at point of care, an analysis of 178 specimens from 147 patients

With the goal of improving patient outcomes by allowing same-day biopsy and treatment, we conducted a clinical trial evaluating the use of Two-photon fluorescence microscopy to rapidly diagnose dermatologic non-melanoma skin cancer biopsies. This study included 178 specimens from 147 patients, achieving high sensitivity and specificity. Furthermore, only a small number of patients would have had treatment differences between two-photon and H&E diagnoses. This suggests that Two-photon fluorescence microscopy has the potential to improve clinical burden and patient outcomes by allowing same-day treatment and diagnosis of low-risk non-melanoma skin cancers.