Intraoperative histology for risk assessment in urology by combining coherent Raman imaging with second harmonic imaging

We present a mobile imaging platform combining Stimulated Raman Scattering (SRS) and Second Harmonic Generation (SHG) microscopy for real-time, label-free diagnostics in urology. SRS provides virtual H&E images for molecular contrast for tumor classification, while SHG highlights collagen structures, aiding interpretation of prostate biopsies in fusion-guided workflows. Our system spans the full Raman spectrum (700–3300 cm⁻¹) and acquires images in milliseconds without staining or sectioning. This enables rapid histological assessment of bladder and prostate tissue with excellent correlation to conventional H&E. The combined SRS-SHG approach enhances intraoperative decision-making and supports more accurate, personalized treatment in urological oncology.