EXAS-OPM enables fast 3D super-resolution imaging via expansion microscopy with aberration-assisted Scheimpflug oblique plane microscopy

EXAS-OPM integrates Expansion Microscopy (ExM) with Aberration-assisted Scheimpflug Oblique Plane Microscopy (AS-OPM) to deliver a highly compact, fast, and cost-effective 3D super-resolution imaging system. By replacing complex correction optics with a single aspheric lens, EXAS-OPM achieves superior stability, simplified alignment, and improved photon efficiency. It offers approximately 125 nm axial and 200 nm lateral resolution at an 8× expansion factor, enabling high-speed 3D super-resolution imaging of entire volumes in just 7 seconds using a standard CMOS camera. With a footprint under 50 × 50 cm, a cost of around $8,000, and rapid assembly, the simplified version of EXAS-OPM combines accessibility with advanced performance. It represents a transformative solution for 3D super-resolution imaging, overcoming the limitations of traditional OPM and light-sheet imaging systems. EXAS-OPM addresses key challenges in light-sheet microscopy and 3D super-resolution imaging, providing a portable and benchtop-friendly alternative for cutting-edge biological imaging.