Estimation of retinal blood velocity variations during moderate intraocular pressure elevation using real-time Doppler holography

We present real-time Doppler holography measurements of systo–diastolic retinal blood velocity variations and arteriovenous pulse delay during moderate intraocular pressure (IOP) elevation. In a healthy subject, IOP was increased from 12.4 ± 0.55 mmHg to 20.4 ± 0.89 mmHg. Automated analysis (EyeFlow v2.6.2, HoloDoppler v2.8) revealed increased arterial resistivity index (0.38 → 0.87), greater venous pulsation depth, and doubling of arteriovenous phase delay (14 → 31 ms). Flow profile widths decreased across the cardiac cycle, consistent with pressure-dependent vessel caliber reduction. These parameters normalized upon IOP recovery. Doppler holography enables beat-to-beat, high-resolution, quantitative assessment of retinal autoregulation and microvascular compliance, offering a reproducible platform for functional vascular diagnostics in ophthalmology and cardiology.