On-glass interference confocal reflectance microscopy for collagen fibril diameter measurement

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing 2026 Open access

Read full text
Authors: Eric P. Hall, Jeffrey W. Ruberti, Charles A. DiMarzio
DOI: 10.1117/12.3081844
Cited by: 0
References: 8

Abstract

Previously, our group demonstrated using interference confocal reflectance microscopy (I-CRM) to measure collagen fibril diameters when suspended. An equally popular but optically unique challenge is presented when imaging collagen on glass, which is used for studying early stage fibril development. In this short manuscript, we present interference confocal reflectance microscopy as an alternative to atomic force microscopy for quantifying collagen fibrils below 50nm.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Collagen: Extraction and Characterization Force Microscopy Techniques and Applications

Publication details

Journal / source
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing
Volume
13857
Pages
9–9
Publication date
4 March 2026
Language
EN