Super-resolved photoluminescence lifetime and intensity mapping of semiconductor quantum dots patterned on DNA origami

Single Molecule Spectroscopy and Superresolution Imaging 2026

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Authors: Bibek Dhakal, Tariq Al-Jarah, María C. Nevárez Martínez, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Christopher M. Green, Sebastián A. Dı́az, Igor L. Medintz, Kimihiro Susumu, Michael H. Stewart, Martin P. Gelfand
+1 more Alan K. Van Orden
DOI: 10.1117/12.3083809
Cited by: 0
References: 17

Abstract

An intensity level-based method for analyzing the emission of single quantum dots and pairs of quantum dots from widefield EMCCD camera images and simultaneously determining their lifetimes using a wide-field time-resolved nocturnal camera (NCam) is discussed. Simulated data is generated by accounting for the probability to image photons from two quantum dots, as well as background emission and dark counts on the pixels of the two detectors. Segregating data based on different intensity levels enables us to localize the emitters with

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Quantum Dots Synthesis And Properties Semiconductor Quantum Structures and Devices

Publication details

Journal / source
Single Molecule Spectroscopy and Superresolution Imaging
Pages
7–7
Publication date
4 March 2026
Language
EN