Quantitative label-free 3D imaging and analysis of live cells and organoids

Advances in three-dimensional (3D) imaging technologies have transformed the ability to study cellular and organoid dynamics in physiologically relevant environments. This work presents a comprehensive approach to quantitative, label-free 3D imaging and analysis of live cells and organoids, leveraging digital holographic tomography and advanced computational reconstruction techniques. Unlike traditional fluorescence-based assays, this method eliminates the need for external dyes or labels, thereby preserving native cellular physiology and enabling long-term, noninvasive observation.