Adaptive cell tracking for intravital 2-photon and 3-photon imaging of real-time cell migration in mouse lymph nodes
Abstract
The performance of multiphoton microscopes is limited by potential tissue heating due to high laser power. We present adaptive cell tracking, which integrates adaptive excitation (illuminating only regions of interest) with real-time cell tracking. Using this method, we demonstrated 3 - 10-fold power reduction for intravital two-photon and three-photon volumetric imaging, which enabled tracking of lymphocytes in mouse lymph nodes over volumes up to 360 × 360 × 180 μm3 at 750 μm depth.
Fields of Study & Subjects
Advanced Fluorescence Microscopy Techniques
Cell Image Analysis Techniques
Nonlinear Optical Materials Studies
Publication details
- Journal / source
- Multiphoton Microscopy in the Biomedical Sciences
- Pages
- 44–44
- Publication date
- 4 March 2026
- Language
- EN