Advances in label-free chemical imaging with stimulated Raman scattering microscopy

Here we present the STELLARIS CRS – Leica’s turnkey Coherent Raman Scattering system. It offers the two popular CRS modalities – Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) – and allows for the simultaneous acquisition of two-photon fluorescence and second-harmonic generation signals. Further, the seamless integration of CRS with the STELLARIS visible confocal fluorescence microscopy platform, as well as Fluorescence Lifetime Imaging, results in a multi-modal optical discovery instrument capable of capturing a unique combination of biochemical, biophysical and molecular contrasts. We highlight new capacities in label-free, multi-modal metabolic profiling at the single-cell level, as well as high-speed multiplex label-free chemical imaging of dynamic biological processes for a wide range of research in the life sciences.