Monooxygenase-dehydrogenase cascade for sustained enzymatic remediation of TMA in salmon protein hydrolysates

ABSTRACT Fish protein hydrolysates hold great promise as nutraceuticals, yet their application as food ingredients or nutraceuticals is currently limited by their fish-like odor. This odor is mainly due to the presence of trimethylamine (TMA), a volatile biogenic amine resulting from the breakdown of naturally occurring trimethylamine-N-oxide (TMAO) in marine fish. The bacterial trimethylamine monooxygenase mFMO can oxidize TMA into TMAO using molecular oxygen and the cofactor nicotinamide adenine dinucleotide phosphate (NADPH). We have established an enzyme cascade that takes advantage of glucose dehydrogenase to recycle NADPH from NADP + , significantly decreasing the cost of the reaction and paving the way for using the enzyme system in fish protein hydrolysates targeted for human consumption. We demonstrate that the dual enzyme system works in an industrially relevant substrate. Salmon protein hydrolysate treated with an mFMO/glucose dehydrogenase cocktail showed a 75% reduction in TMA. A trained sensory panel perceived an improved odor across several parameters, including a reduction in the characteristic TMA smell. IMPORTANCE Marine by-products are a valuable source of high-quality peptide ingredients; however, their application in the food market is limited by the unpleasant fishy odor caused by trimethylamine (TMA). An enzyme that oxidizes TMA to the odor-free trimethylamine-N-oxide (TMAO) in salmon protein hydrolysates is known, but it requires excessive amounts of NADPH, an expensive cofactor. Here, we describe a cofactor regeneration system that allows using less cofactor in the enzyme-driven TMA removal process. This dual enzyme system removed 75% of TMA from a salmon protein hydrolysate, resulting in a significantly reduced fishy odor as confirmed by a trained sensory panel compared to the untreated control. This enzyme cascade is an important step toward making targeted TMA removal economically feasible for marine biomass valorization.