Loss of Pol32, a subunit of DNA polymerases δ and ζ, leads to different patterns of genome stability than direct impairment of these individual polymerases

mBio 2026 Open access

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Authors: Lei Qi, Ke-Jing Li, Xing-Xing Tang, Li-Yan Tian, Yeke Wang, Min He, Ke Zhang, Thomas D. Petes, Dao‐Qiong Zheng
DOI: 10.1128/mbio.00531-26
Cited by: 1
References: 44

Abstract

ABSTRACT Pol32 is a subunit shared by DNA polymerases δ and ζ, yet its role in maintaining genome integrity remains incompletely defined. Here, we employed whole-genome sequencing of mutation-accumulation lines to systematically characterize the genome-wide effects of a POL32 deletion in diploid Saccharomyces cerevisiae . Loss of Pol32 led to substantially (>5-fold) elevated rates of loss of heterozygosity (LOH), chromosome rearrangements, and aneuploidy, but resulted in substantially less genome instability than observed in strains with low levels of DNA polymerase δ. In particular, there was only a small (

Fields of Study & Subjects

DNA Repair Mechanisms Genetic factors in colorectal cancer Microtubule and mitosis dynamics

Publication details

Journal / source
mBio
Publisher
American Society for Microbiology
Volume
17
Issue
5
Pages
e0053126–e0053126
Publication date
17 April 2026
Language
EN