AmpliFuse: an amplicon simulation tool with enhanced chimera generation for Illumina platforms
Abstract
ABSTRACT PCR-generated chimeric amplicons undermine the accuracy and reliability of subsequent amplicon-sequencing analyses. AmpliFuse, a Python-based tool, simulates realistic amplicon datasets through in silico PCR, chimera formation, and read simulation. By providing biologically relevant chimera-containing amplicon reads, AmpliFuse would facilitate benchmarking of chimera-detection algorithms and workflows, advancing microbiome and AMR research.
Fields of Study & Subjects
Genomics and Phylogenetic Studies
Microbial Community Ecology and Physiology
Environmental DNA in Biodiversity Studies
Publication details
- Journal / source
- Microbiology Resource Announcements
- Volume
- 15
- Issue
- 2
- Pages
- e0143925–e0143925
- Publication date
- 27 January 2026
- Language
- EN