Oxford Nanopore enhanced accuracy of long-read amplicons applied to microbial whole-genome sequencing
Abstract
The development of long-read sequencing technologies has enabled the analysis of extended nucleic acid sequences. These methods have proven their strength through their capacity to generate long reads, facilitating the analysis of complex genomic regions and rearrangements. Oxford Nanopore Technologies (ONT) offers a rapid and portable system that brings sequencing to the field. Although this is a great advantage for clinical settings, applications of long-read sequencing in this context have been limited by the high error rates reported for these methods. Here, we report an adaptation of an amplicon sequencing approach combined with unique molecular identifiers. We applied this method to whole-genome sequencing using mock community samples and human blood cultures spiked with common bloodstream infection pathogens. Our results showed a total error rate of
Fields of Study & Subjects
Genomics and Phylogenetic Studies
Burkholderia infections and melioidosis
Bacterial Identification and Susceptibility Testing
Publication details
- Journal / source
- Microbiology Spectrum
- Publisher
- American Society for Microbiology
- Volume
- 14
- Issue
- 4
- Pages
- e0285625–e0285625
- Publication date
- 10 March 2026
- Language
- EN