GFP reporter system reveals cell-to-cell variability in aquaporin-2 expression
Abstract
To investigate regulatory mechanisms in Aqp2 gene transcription, we engineered an Aqp2 reporter cell line using CRISPR/Cas9 to insert a green fluorescent protein (GFP) cassette at the endogenous Aqp2 gene locus in mpkCCD cells. We demonstrate that the GFP reporter accurately and dynamically tracks the expression and regulation of endogenous Aqp2. We reveal that Aqp2 heterogeneity in mpkCCD cells is at least partly driven by differences in cell cycle phase.
Fields of Study & Subjects
Ion Transport and Channel Regulation
Congenital heart defects research
Physiological and biochemical adaptations
Publication details
- Journal / source
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Volume
- 330
- Issue
- 4
- Pages
- C782–C793
- Publication date
- 24 February 2026
- Language
- EN