The gut microbiota metabolite isovalerate enhances the epithelial barrier function in cell monolayers derived from porcine ileum organoids

American Journal of Physiology-Gastrointestinal and Liver Physiology 2026

View at publisher
Authors: Martin Beaumont, Cláudia M. Vicente, Cristina Plata-Calzado, Corinne Lencina, Elisabeth Jones, Stéphanie Lecuelle, Tristan Chalvon‐Demersay
DOI: 10.1152/ajpgi.00193.2025
Cited by: 6
References: 64

Abstract

The microbiota degrades branched-chain amino acids to produce branched-chain fatty acids, the effects of which on the intestinal epithelial barrier remain poorly described. In this study, we demonstrate that the leucine-derived metabolite isovalerate enhances epithelial barrier function in cell monolayers derived from porcine ileum organoids. We also identified gut microbiota species potentially able to produce branched-chain fatty acids that could be targeted to promote gut health.

Fields of Study & Subjects

Gut microbiota and health Barrier Structure and Function Studies Clostridium difficile and Clostridium perfringens research

Publication details

Journal / source
American Journal of Physiology-Gastrointestinal and Liver Physiology
Publisher
American Physiological Society
Volume
330
Issue
4
Pages
G459–G477
Publication date
25 February 2026
Language
EN