Sex-dependent differences in bioenergetics of young mouse brain microvasculature: implications for oxygen-glucose deprivation and reoxygenation injury

American Journal of Physiology-Heart and Circulatory Physiology 2026 Open access

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Authors: Venkata N. Sure, Lokanatha Oruganti, Siva SVP. Sakamuri, Swathi Chitra Pasupulati, Raed Y. Ageeli, Partha Chandra, Ibolya Rutkai, Xiaoying Wang, Sarah H. Lindsey, Ricardo Mostany, David W. Busija, Prasad V.G. Katakam
DOI: 10.1152/ajpheart.00195.2025
Cited by: 3
References: 97

Abstract

Impact of sex on microvascular bioenergetics has never been studied. We measured oxygen consumption rates and extracellular acidification rates in mouse brain microvessels using Agilent Seahorse XFe24 analyzer. For the first time, we observed decreased mitochondrial respiratory and glycolytic function in brain microvessels of female mice compared with male mice. These sex-dependent differences in glycolysis and oxidative phosphorylation may increase the vulnerability/protection of the microvasculature to vascular cell injury.

Fields of Study & Subjects

Barrier Structure and Function Studies Mitochondrial Function and Pathology Cancer Hypoxia and Metabolism

Publication details

Journal / source
American Journal of Physiology-Heart and Circulatory Physiology
Publisher
American Physiological Society
Volume
330
Issue
3
Pages
H671–H685
Publication date
12 January 2026
Language
EN