Estimation of flow-induced deformation of glycocalyx layer on vascular endothelial cells: comparison of three methods
Abstract
Endothelial glycocalyx layer deformation under physiological fluid flow remains to be understood. This study provides the first direct quantification of shear-induced deformation of the endothelial glycocalyx layer using three distinct confocal microscopic approaches: photobleaching, thickness measurement, and quantum dots imaging. The observed shear strains from three approaches were consistent (3-10°/Pa), revealing that the glycocalyx is actually deformed in response to fluid shear stress and may serve as a key mechanosensor in vascular endothelial cells.
Fields of Study & Subjects
Trauma
Hemostasis
Coagulopathy
Resuscitation
Blood properties and coagulation
Cell Adhesion Molecules Research
Publication details
- Journal / source
- American Journal of Physiology-Heart and Circulatory Physiology
- Publisher
- American Physiological Society
- Volume
- 330
- Issue
- 4
- Pages
- H1117–H1128
- Publication date
- 27 February 2026
- Language
- EN