Rostafuroxin ameliorates cardiac glycoside-induced cardiomyocyte electrolyte imbalances and arrhythmia in ovo

American Journal of Physiology-Heart and Circulatory Physiology 2026 Open access

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Authors: Judith Rees, Annika Winkler, Judith Huettemeister, Laura Stengel, Paul Spangler, Girish Ramesh, J. J. P. v. d. Kamp, Teresa Charlotte Funk-Hilsdorf, Laura Michalick, Anna-Lena Herm, Peter Maximilian Deissler, Alexandr Melnikov
+8 more Michael Höpfner, Felix Hohendanner, Claudia Crocini, Callum Zgierski Johnston, Steffen Pabel, Bianca Nitzsche, Jana Grune, Niklas Hegemann
DOI: 10.1152/ajpheart.00652.2025
Cited by: 1
References: 51

Abstract

This study evaluates rostafuroxin, a selective ouabain inhibitor, for its potential to antagonize electrophysiological derangements in ouabain overdosing. Methodologically, the study uses the iCE model, previously introduced as a suitable 3 R-compliant cardiovascular research platform. We developed and validated a comprehensive electrophysiological workflow in iCEs to perform our investigations. Ouabain increased heart rate variability, induced arrhythmia and electrolyte imbalances in iCEs, whereas rostafuroxin largely protected them from these effects.

Fields of Study & Subjects

Cardiac electrophysiology and arrhythmias Poisoning and overdose treatments Ion Transport and Channel Regulation

Publication details

Journal / source
American Journal of Physiology-Heart and Circulatory Physiology
Publisher
American Physiological Society
Volume
330
Issue
3
Pages
H838–H853
Publication date
5 February 2026
Language
EN