Single-cell microfluidic quantification of miRNA-21 and miRNA-34a reveals miRNA interactions in small airway epithelial cells and fibroblasts from patients with COPD

American Journal of Physiology-Lung Cellular and Molecular Physiology 2026 Open access

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Authors: Vanessa Ho, Jonathan R. Baker, Peter S Fenwick, K R Willison, D R Klug, Peter J. Barnes, Louise Donnelly
DOI: 10.1152/ajplung.00292.2025
Cited by: 1
References: 39

Abstract

This study demonstrates a novel microfluidic system that can precisely quantify two specific microRNAs in COPD samples at a single-cell resolution using small quantities of cells and reveal the cellular heterogeneity in cell populations. This dual detection system enables the interrelationship between microRNAs in the same cell to be studied as well as having the ability to monitor microRNA levels during disease progression and therapeutics.

Fields of Study & Subjects

MicroRNA in disease regulation Extracellular vesicles in disease Chronic Obstructive Pulmonary Disease (COPD) Research

Publication details

Journal / source
American Journal of Physiology-Lung Cellular and Molecular Physiology
Publisher
American Physiological Society
Volume
330
Issue
3
Pages
L253–L265
Publication date
13 February 2026
Language
EN