Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid

American Journal of Physiology-Lung Cellular and Molecular Physiology 2026 Open access

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Authors: Taryn B. Gellner, Belinda Chen, Shreyas R. Raini, M. L. Jackson, Amy K. Kraak, Sophie C. Petta, Margherita Paschini, Lynn M. Schnapp, Carla F. Kim, Monica Yun Liu
DOI: 10.1152/ajplung.00356.2025
Cited by: 1
References: 48

Abstract

We provide streamlined protocols to generate both airway and alveolar epithelial organoids from a single, clinical BAL specimen. From standardized specimen collection to organoid plating to passaging and differentiation, we show that rare, primary epithelial cells in BAL can give rise to all the major airway and alveolar cell types. Our serum-free, feeder-free, and sorting-free methods offer a simplified starting point for using patient-derived organoids to model lung disease.

Fields of Study & Subjects

Neonatal Respiratory Health Research Cancer Cells and Metastasis Inhalation and Respiratory Drug Delivery

Publication details

Journal / source
American Journal of Physiology-Lung Cellular and Molecular Physiology
Publisher
American Physiological Society
Volume
330
Issue
5
Pages
L576–L592
Publication date
30 March 2026
Language
EN