Differential expression of miRNAs in slow and fast muscle fibers isolated from GFP-Myh7 mice
Abstract
Skeletal muscle comprises fast and slow fiber types, which reflect physiological and metabolic features. Although identifying fiber types without PCR or antibody-based assays was challenging, we visually isolated slow- and fast-type fibers from GFP-Myh7 mice, in which slow-type fibers express green fluorescent protein (GFP). Using these mice, we successfully profiled miRNA expression in precisely distinguished slow- and fast-type fibers to capture fiber-type-dependent miRNA expression.
Fields of Study & Subjects
Muscle Physiology and Disorders
MicroRNA in disease regulation
Circular RNAs in diseases
Publication details
- Journal / source
- Physiological Genomics
- Publisher
- American Physiological Society
- Volume
- 58
- Issue
- 2
- Pages
- 115–123
- Publication date
- 14 January 2026
- Language
- EN