High-Throughput and Dynamic Kidney DECM-based Miniaturized 3D Tissue Culture Platform

The kidney’s limited regenerative capacity poses a major challenge for modeling renal diseases and developing effective therapeutics. To address this limitation, a kidney-derived decellularized extracellular matrix methacrylate (KdECMMA) bioink and a high-throughput droplet-based bioprinting platform were developed to support kidney organoid culture. The KdECMMA bioink was synthesized to recapitulate the biochemical complexity of native kidney tissue, providing a physiologically relevant microenvironment. Using the developed printing system, uniform and reproducible hydrogel droplets were fabricated for organoid culture under both static and dynamic conditions incorporating air–liquid interface (ALI) with gentle rocking-based convection. Cell viability exceeded 90% under all conditions, confirming cytocompatibility of the printing and culture environments. Dynamic culture significantly enhanced cell morphology and cluster formation compared to static culture, suggesting enhanced organoid-like cluster formation. This integrated platform establishes a foundation for reproducible and scalable kidney organoid culture.