Supplementary Figure S6 from Stress Promotes Lung Metastasis in Breast Cancer by Altering Neutrophil Differentiation

Clustering analysis of T cells in the lungs of NC and CS MMTV-PyMT mice. A. UMAP plot showing different populations of T cells from the single-cell RNA sequencing analysis of the lung. B. Comparison of the fraction of each T cell subpopulation between the CS and NC groups. (Fisher exact test, *p < 0.005, **p < 0.01, ***p < 0.001). C. Dot plots showing marker genes for each subpopulation of T cells. D. Flow cytometry analysis of Treg (stained with CD25 and FOXP3) in the lungs of mice with or without receiving chronic stress stimulation. E. Quantitative analysis of Treg cells in D (T test). F-H. ModuleScore analysis of genes in T cells regulating T cell proliferation pathway (F), T cell activation pathway (G), or cell killing pathway (H), respectively. Comparisons were performed between NC and CS mice (T test). CS: chronic stress. NC: negative control.