Figure S6 from SPP1 Drives Colorectal Cancer Liver Metastasis and Immunotherapy Resistance by Stimulating CXCL12 Production in Cancer-Associated Fibroblasts

Figure S6 Talabostat mesylate reverses the progression of CRLM and synergizes with PD-1 blockade therapy A-C Collagen content assessed by Masson’s trichrome staining, and expression levels of α-SMA and CD8 evaluated by mIHC analysis. D, E Ki-67 and TUNEL staining were performed on PDOX tumor tissues to evaluate cell apoptosis and proliferation. F H&E analysis in tumor tissues from each group. G Flow cytometry validated the engraftment of hCD45+ cells 14 days post implantation in each group of mice (n = 3/group). H Collagen content was assessed using Masson’s trichrome staining, while expression levels of α-SMA and CD8 were evaluated through mIHC analysis. Ki-67 and TUNEL staining were performed on PDX tumor tissues to assess cell apoptosis and proliferation. H&E analysis was conducted on tumor tissues from each group. I, J Collagen content assessed by Masson’s trichrome staining, and expression levels of α-SMA evaluated by IHC analysis. IOD: Integrated Optical Density. K TUNEL staining was performed to evaluate cell apoptosis. All results are presented as mean ± SEM. P values were determined by one-way ANOVA. **P < 0.01, ***P < 0.001.