Figure S3 from SPP1 Drives Colorectal Cancer Liver Metastasis and Immunotherapy Resistance by Stimulating CXCL12 Production in Cancer-Associated Fibroblasts

Figure S3 SPP1 positively correlates with CAF marker expression and promotes FAP expression. A Correlation analysis of SPP1 with CAF marker genes from TCGA data. B Brightfield and fluorescence images of PT-PDOs #1 and LM-PDO #1 stably expressing GFP-SPP1. Green fluorescence indicates SPP1 expression. Scale bar, 500 μm. C Supernatants from PDOs overexpressing SPP1 or Vector were collected at various time points to stimulate CAFs, followed by western blot analysis of FAP level, n = 3 biologically independent experiments. CM, Conditional Medium.