Figure S1 from SPP1 Drives Colorectal Cancer Liver Metastasis and Immunotherapy Resistance by Stimulating CXCL12 Production in Cancer-Associated Fibroblasts

Figure S1 Verification of high-metastasis mouse model and the relationship between SPP1 and clinical features. A-F Results of bright field imaging, migration, and invasion assays for LoVo, LoVo-HM, HCT116 and HCT116-HM cells are presented, n = 3 biologically independent experiments. Scale bar, 50 μm. G-J Representative metastatic livers gross morphology from NOD/SCID mice intrasplenically implanted with LoVo, LoVo-HM, HCT116 or HCT116-HM cells (n = 5 per group). K Western blot analysis of SPP1 protein levels in LoVo, LoVo-HM, HCT116, and HCT116-HM cells, n = 3 biologically independent experiments. L Expression of SPP1 gene in different cancer and normal tissues of TIMER2.0 database. M, N Association of SPP1 expression with OS (M) and DFS (N) in CRC based on TCGA data. O-Q Correlation of SPP1 expression with pathological stage, N stage and T stage in CRC patients. All results are presented as mean ± SEM. The data in M, N were determined by Kaplan–Meier analysis with the log-rank test. P values were determined by two-tailed unpaired Student’s t test (C-F, H, J, P) and one-way ANOVA (O, Q). **P < 0.01, ***P < 0.001.