Supplemental Figure S7 from Stress Promotes Lung Metastasis in Breast Cancer by Altering Neutrophil Differentiation

scRNA-seq analysis of MoMacDc cell subpopulations in the lung of NC and CS MMTV-PyMT mice. A. UMAP plot showing different population of MoMacDc cells from the single-cell RNA sequencing analysis of the lung. B. Comparison of the fraction of each MoMacDc cell subpopulation between the CS and NC groups. (Fisher exact test, ***p < 0.001). C. Heatmap showing the expression of marker genes in each subpopulation of MoMacDc cells. D. Flow cytometry analysis of Macrophages (stained with F4/80) in the of lung of mice with or without receiving chronic stress stimulation. E. Quantitative analysis of Macrophages in D (T test). F. Volcano plot displaying differentially expressed genes (DEGs) in dendritic cells between the CS and NC groups. Those MHC Class II Protein Complex-related genes were highlighted in red. G. Bubble plot displaying the top 10 significantly downregulated pathways (NES