Figure 2 from Mitochondrial Transfer Rescues Respiration to Support De Novo Pyrimidine Biosynthesis and Tumor Progression

Absence of DHODH suppresses efficient tumor growth. A, Expression of DHODH in parental and DHODHKO cells (HCT116, MDA-MB-231, and PaTu 8902 cell lines) was assessed by Western blotting analysis. B–D, Routine and DHODH-dependent respiration was evaluated in parental and DHODHKO cells (B and C), as well as their proliferation (number of cells; D) at 72 hours in the presence and absence of uridine in the media. E, NSG mice were grafted subcutaneously with parental and DHODHKO cells (106 per animal), and tumor volume was assessed using calipers (n = 6 for each group). The experiments are representative of at least three independent repetitions (n ≥ 3). Statistical analysis was performed using an unpaired t test with GraphPad Prism 8 software, considering differences with a P value of ≤ 0.05 as statistically significant. Images are representative of three independent experiments of three separate biological replicates. *, P value of 0.01 to 0.05; **, P value of 0.001 to 0.01.