Figure S1 from Protumor Macrophage Polarization Mediated by BMP2/4–ACVR1 Signaling Orchestrates an Immunosuppressive Microenvironment during Tumor Initiation

Extended Data Fig.1. EGFR driven lung adenocarcinoma formation, Related to Figure 1 (A) Schematics of the experimental design for lung cancer induction with doxycycline. Lung tissues were collected for scRNA-seq from mice fed with doxycycline for 0, 1, 2, 4, and 8 weeks. (B) H&E staining images of lung sections from mice fed with doxycycline at the indicated time points, highlighting a rapid increase in overall cell density in the mouse lung. Scale bar :50um. (C) Longitudinal MRI imaging of mouse lungs during tumor induction at 0, 1, 2, 4, 6, and 8 weeks. The serial images illustrate the progressive development of lung adenocarcinoma features over time. (D) Immunofluorescence staining for EGFR (green), Ki67 (red) along with DNA visualized by DAPI staining. Scale bar : 50um.