Figure S4 from PAK5-Mediated Suppression of β-Hydroxybutyrate Production Promotes Breast Cancer Metastasis and Can Be Overcome with Ketogenic Diet

Supplementary Figure 4. Treatment with both a PAK5 inhibitor (C55) and β-HB suppresses the growth, migration, and invasion of breast cancer cells (a)CCK8 proliferation analysis of MDA-MB-231 cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB (10mmol/L) or a combination of C55 and β-HB, the data were presented as a histogram of the mean and SEM of three independent experiments, ****P < 0.0001. (b)Colony formation analysis of MDA-MB-231 upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB, representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. ***P < 0.001, ****P < 0.0001. (c)Wound-healing analysis of MDA-MB-231 cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB. Results were representative of three independent experiments. ****P < 0.0001. (d)Transwell analysis of MDA-MB-231 cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB. Results were representative of three independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001. (e)CCK8 proliferation analysis of HS578T cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB, the data were presented as a histogram of the mean and SEM of three independent experiments, ****P < 0.0001. (f)Colony formation analysis of HS578T upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB, representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. ***P < 0.001, ****P < 0.0001. (g)Wound-healing analysis of HS578T cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB. Results were representative of three independent experiments. ***P < 0.001, ****P < 0.0001. (h)Transwell analysis of HS578T cells upon treatment with PAK5 inhibitor (C55, 10μmol/L), β-HB(10mmol/L) or a combination of C55 and β-HB. Results were representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.