Supplementary Figure S5 from Targeting AEBP1 to Mitigate Protumor Activity of Cancer-Associated Fibroblasts and Increase Therapeutic Efficacy of Anti–PD-1

Supplementary figure S5 shows the scRNA-Seq analysis of MC38 tumor cells isolated from co-injection model (A) Tumor volumes on the day of tumor harvest for single cell RNA-seq. (B) 2D UMAP of integrated single cell RNA-seq data of all tumor samples. Tumor cells, immune cells and CAF clusters were identified and labeled by marker gene expression. The MC38 tumor cluster of interest is marked by a circle. (C) 2D UMAP of sub-clustering of single cell RNA-seq of the MC38 tumor cluster. (D) Scaled expression of top cluster identifying genes from each tumor sub-cluster. (E-H) Top 15 enriched pathways of top 100 cluster identifying genes for tumor sub-cluster 0, 1, 3, and 4. The significance of enrichment is identified by hypergeometric tests against MsigDB pathway gene sets. Significant (adjusted p-value