Supplementary Movie S1 from A Plastic EMP1+ to LGR5+ Cell State Conversion as a Bypass to KRASG12D Pharmacologic Inhibition in Metastatic Colorectal Cancer

Time-lapse imaging of representative AKTP MTO organoids from vehicle and RMC-9945 treatment conditions. Initial imaging (time 0h) was performed prior to vehicle or RMC-9945 treatment. Upon treatment, organoids were imaged every hour over a 48-hour period. Top (vehicle) and bottom (RMC-9945) panels show XY planes positioned at three z-depths relative to the centre of the organoids volume (-10 µm, 0 µm and +10 µm). Lgr5-EGFP is shown in green and Emp1-TOM in red. Scale bar, 50 µm.