Figure S4 from Targeting SALL4 with an HLA Class I–Restricted TCR for Cancer Immunotherapy

SALL4-derived S9V specific CD8+ T clone recognize and kill MDA-MB231-SALL4 cell line in vitro. Mean number of IFN-γ (left) and GZMB (right) spots counted on ELISpot upon 24h coculture of SALL4 specific CD8+ T clone with MDA-MB231-SALL4 with different E:T ratio 10:1, 5:1, 1:1, 1:5 and 1:10. Data represent mean ± SD of duplicates. Responses were considered positive if the number of SFC was > 10 spots/104 T clone and 2 times the background value. MDA-MB231-CTRL cell line transduced with empty lentiviral vector was used as negative control. Anti MHC-I antibody was used for specific T cell restriction reactivation validation.