Figure S10 from Targeting SALL4 with an HLA Class I–Restricted TCR for Cancer Immunotherapy

SALL4-TCR T cells do not recognize human hematopoietic stem cells CD34+ in vivo. (A) Schematic outlining the experimental procedure of adoptive T cell transfer in hNSG mice. hNSG mice were engrafted IV with 107 Mock (n=9) or SALL4-TCR transduced T cells (n=10) or not (n=8). Seven days after injection, mice were sacrificed and BM were collected. Blood samples were harvested at day 0 before T cells injection and at day 7 following T cell injection. (B) Flow cytometry gating strategy in BM (left) and in the blood samples (right). (left) HSC were determined by the expression of CD34 and CD38 among human CD45 cells. Dendritic cell (DC), in pink, were determined by the medium expression on CD33 and medium expression of CD123, HLA-DR and CD303. Monocytes, in blue, were determined by the high expression of CD33, medium expression on HLA-DR, CD123 and CD303. (right) The percentage of humanization is determined by the ratio mCD45/hCD45. Monocytes were selected from human cells (hCD45+) based on the expression of CD33 and CD14. (C) and (D) Flow cytometry analyses of HSC and myeloid-derived differentiated cells in BM (C) and monocytes in blood (D) among hCD45 cells.