Supplemental Figure 1 from T-cell Priming by High-Avidity Neoantigens in Lymph Nodes Augments Adoptive Immunotherapy

Supplemental Figure 1: Quality assurance by flow cytometry of naïve cytotoxic T cells prior to adoptive transfer to mice bearing melanoma. (A) Gating Strategy for isolating lymphocytes followed by selection of singlets and subsequently live cells (B) Double positive Vβ13+ Thy1.1+ cells were gated on live cells (C) CD44 and CD62L characterization of Vβ13+ Thy1.1+ was used to assess efficacy of naïve T cell isolation (>94% yield).