Supplementary Figures from Enhancer Hijacking Discovery in Acute Myeloid Leukemia by Pyjacker Identifies MNX1 Activation via Deletion 7q

Supplementary Figure 1. Summary of the somatic alterations in the 39 ckAML samples. Supplementary Figure 2. Proportion of samples expressing the top pyjacker hits, for several AML cohorts profiled with RNA-seq. Supplementary Figure 3. Example rearrangements leading to gene activation and TP53 inactivation. Supplementary Figure 4. Rearrangements leading to MECOM monoallelic expression in sample 15KM20146. Supplementary Figure 5. Validation of the translocation t(1;3)(p36;q21) in sample 16KM11270 and of the breakpoints for the CDK6 enhancer duplication next to MNX1 in sample 15PB8708 by genomic PCR. Supplementary Figure 6. Deletion between the TCR beta locus and MNX1 leading to MNX1 expression. Supplementary Figure 7. Alternative rearrangements leading to MNX1 expression. Supplementary Figure 8. Differential gene expression analysis of MNX1 status in adult and pediatric AML. Supplementary Figure 9. Gene expressions of 25 selected cancer and hematological development associated genes differentially expressed under MNX1 activation. Supplementary Figure 10. Copy number alterations on chromosome 7 for samples profiled with scRNA-seq. Supplementary Figure 11. Reciprocal 4C. Supplementary Figure 12: Knockdown of MNX1 reduces tumor load of AML PDX cells in vivo.