Figure 5 from CD70-Targeting CAR NK Cells Overcome BCMA Downregulation and Improve Survival in High-risk Multiple Myeloma Models

Loss of BCMA does not affect the anti-myeloma activity of CAR27/IL-15 NK cells. A, Whole-cell lysates from K562 (negative control), MM.1S wild-type (WT), and MM.1S TNFRSF17 KO cells (KO) were analyzed by Western blotting for BCMA expression. β-actin served as the loading control. A representative blot is shown. B,TNFRSF17 KO efficiency analyzed by flow cytometry. C, CD70 expression of MM.1S WT and MM.1S TNFRSF17 KO tumor cells. D,51Cr release assay of NT NK cells or CAR27/IL-15 NK cells against MM.1S TNFRSF17 KO cells. E, Incucyte real-time killing assay of MM.1S TNFRSF17 KO tumor cells by NT NK, IL-15 NK, and CAR27/IL-15 NK cells. Bar graph (right) shows the AUC analysis. Data are represented as mean ± SD. Data for the AUC graph are represented as mean ± SEM. The P values were determined by two-way ANOVA with Sidak’s multiple comparisons (D) and one-way ANOVA with Tukey multiple comparisons (E). ***, P ≤ 0.001.