Figure 5 from Cell State Transitions Drive the Evolution of Disease Progression in B-Lymphoblastic Leukemia

CD34+/CD38− self-renewal rate correlates with BCR::ABL1 status and MRD. A, PCA of all Markov transition rate parameters, Mij, color-coded by BCR::ABL1 status. Arrows indicate the loadings for each parameter, representing the correlation between the variables and the principal components. BCR::ABL1-positive patients tend to cluster near loadings for transition parameters back into the stem cell state, M11, M21, M31, and M41. B, Waterfall plot for patients in rank order by the value of stem cell self-renewal transition rate, M11. C, Violin plot for self-renewal rate, M11, categorized by BCR::ABL1 status, showing statistically different values. D, PCA color-coded by MRD outcomes. E, Waterfall plot rank ordered by stem self-renewal transition rate, M11, color-coded by MRD outcome. F, Violin plot for stem self-renewal transition rate, M11, color-coded by MRD outcome. G, PCA color-coded by relapse. H, Waterfall plot rank ordered by stem cell self-renewal transition rate, M11, color-coded by relapse. I, Violin plot for stem cell self-renewal transition rate, M11, color-coded by relapse. The percentage of variance explained differs between panels because not all patients had MRD or relapse data available, and thus, PCA was performed on slightly different patient subsets. *, Statistical significance using two-sample t test with significance value of 0.05.