Figure 7 from OPG-Producing B Cells and RANKL-Expressing T Cells Define Immune Signatures Predictive of Bone Metastases in Breast Cancer

Tumor-infiltrating CD4+ T and CD19+ B cells from 4T1 and 67NR tumor–bearing mice exhibit distinct RANKL and OPG profiles. A, mRNA expression of Tnfsf11 (RANKL) and Tnfrsf11b (OPG) in CD4+ T and CD19+ B cells isolated from tumors and LNs of mice bearing 4T1 or 67NR tumors, evaluated by qRT-PCR. Results are normalized and expressed as fold change relative to naive controls. B, Quantification of secreted RANKL and OPG in culture supernatants of CD3+ CD4+ T cells (left) and CD19+ B cells (right) restimulated with 4T1 tumor sAg by ELISA. Data are presented as mean ± SD and are representative of two independent experiments (n = 5 mice per group). Statistical significance was considered at P < 0.05. Statistical comparisons between more than two groups were performed using one-way ANOVA followed by Tukey post hoc test for pairwise comparisons when data were normally distributed.