Figure 2 from The Lysine Demethylase KDM4C Is an Oncogenic Driver and Regulates ERK Activity in KRAS-Mutant Pancreatic Ductal Adenocarcinoma

CRISPR-mediated KDM4C deletion attenuates PDAC growth in vitro and in vivo. A, Western blot showing KDM4C depletion in selected AsPC1 and KPC-mT4 KDM4C-null clones. B, Colony formation assay (CFA): representative wells comparing AsPC1 cells to KDM4C KO isogenic cells show reduced colony formation ability in the absence of KDM4C. C, Quantification of CFA by ImageJ shows a significant reduction in the number of colonies in KDM4C KO cells compared with KDM4C WT cells; P value was determined by one-way ANOVA test; *, P ≤ 0.05; **, P ≤ 0.01. D, Proliferation assay measured through % confluence over time by the Incucyte Live Imaging system shows decreased proliferation in the absence of KDM4C, P value determined by one-way ANOVA test. E, Kaplan–Meier survival plot showing increased survival of B6 mice orthotopically transplanted with KDM4C KO KPC-mT4 cells compared with those transplanted with KDM4C WT KPC-mT4 (n = 6 per arm).