Figure S3 from Comparative Analysis of Cell-Free DNA Fragmentation Patterns in Canines with Sarcoma and Tumor-Free Canines and Humans

Evaluation of pre-analytical variables in canine cfDNA collection and processing. A. Comparison of DNA concentration by qPCR (LINE99) between the first and second blood tubes collected from each dog (n=18, paired p = 0.38). B. Comparison of fraction of short fragments measured by qPCR (short index) between the first and second blood tubes collected from each dog (n=18 pairs, paired p = 0.22). C. Comparison of DNA concentration by qPCR (LINE99) between samples processed by two different operators in two different lab environments (n=6 for laboratory A and n=281 for laboratory B, unpaired p = 0.12) D. Comparison of fraction of short fragments measure by qPCR (short index) between samples processed by two different operators in two different lab environments (n=6 for laboratory A and n=281 for laboratory B, unpaired p = 0.98).