Figure 1 from Comparative Analysis of Cell-Free DNA Fragmentation Patterns in Canines with Sarcoma and Tumor-Free Canines and Humans

Comparison of cfDNA fragment size distributions in dogs and humans. A, Median human and canine cfDNA fragment lengths measured by electrophoresis. Dogs have a significantly higher proportion of longer DNA fragments. B, Proportion of short DNA fragments determined by the ratio of amplicons with different lengths in a multiplexed qPCR assay. C, Fragment size distributions of human and canine cfDNA derived from short-read sequencing. D, Fragment size distributions of human and canine cfDNA derived from long-read cfDNA sequencing. Four samples were analyzed including cfDNA extracted from commercially obtained human (blue) and canine (dark purple) plasma samples, as well as plasma samples from two healthy dogs enrolled at our institution (light purple). The inset shows the 600–5,000 bp range in detail. E, Cumulative proportion of fragment sizes from long-read sequencing shown in D.